Supplemental Data Zfp423/OAZ Participates in a Developmental Switch during Olfactory Neurogenesis

Supplemental Experimental Procedures OAZ-lacZ Gene-Trap Clone XB409 The ES cell clone was obtained from Baygenomics (www. baygenomics. ucsf.edu), the site of genomic insertion and fusion of OAZ gene with lacZ was confirmed by RT-PCR from ES RNA, and injected into C57BL/6 blastocysts. The genomic insertion site was mapped by genomic southern blot and confirmed by sequencing. After germ line transmission, the heterozygous OAZ mice were backcrossed with C57BL/6, and the line was maintained either by heterozygous intercrosses or by heterozygous backcrossing to C57BL/6 mice. Homozygous OAZ pups were born, but died within one day of birth, indicating the insertion also disrupts OAZ function.